ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis of bladder cancer cell 5637 induced by pseudolaric acid B in vitro and its mechanism.</p><p><b>METHOD</b>The cell proliferation was detected by MTT assay;the cell cycle was measured by flow cytometry; the cell apoptosis was observed by flow cytometry with Annexin V-FITC/PI double staining; the expressions of survivin protein and caspase-3 protein were detected by Western blot assay.</p><p><b>RESULT</b>It showed that pseudolaric acid B remarkably induced apoptosis of 5637 cell line. Moreover, pseudolaric acid B suppressed survivin and up-regulated caspase-3 expression.</p><p><b>CONCLUSION</b>Pseudolaric acid B inhibits the proliferation and induces the apoptosis of 5637 cells. The molecular mechanism of pseudolaric acid B inducing the apoptosis of 5637 cells may be associated with its action of down-regulating the expression of survivin, and up-regulating the expression of caspase-3.</p>
Subject(s)
Humans , Apoptosis , Caspase 3 , Cell Cycle , Cell Line, Tumor , Cell Proliferation , Diterpenes , Pharmacology , Drugs, Chinese Herbal , Pharmacology , Inhibitor of Apoptosis Proteins , Urinary Bladder Neoplasms , Drug Therapy , PathologyABSTRACT
BACKGROUND:Recent investigations show that there are a small part of self-renewing and multi-potent cells named as tumor stem cells.Those cells share many characteristics with somatic and embryonic stem cells and are thought to be responsible for driving tumor progression in growing list of neoplastic diseases.Vascular endothelial growth factor(VEGF)is an important angiogenesis factor,which can regulate endothelial proliferation,angiogenesis,permeability of blood vessel,and thrombogenesis.OBJECTIVE:To summarize the progress of cancer stem cells and tumor angiogenesis.METHODS:A computer-based online search was conducted in PUMMED,CNKI,and Wanfang databases with the key words of "cancer stem cells,vascular endothelial growth factor,new vascularize" in both Chinese and English from January 2000 to October 2009.RESULTS AND CONCLUSION:Among 153 articles,there were 13 in Chinese and 140 in English.Titles and abstracts were preliminarily screened,and articles which were non-relative(n=35),duplicated(n=30),and Meta analysis(n=57),were excluded.A total of 31 articles were included in the final analysis.Angiogenesis regulated by multiple factors was a necessary link for tumor growth and transferring.Stem cells promoted angiogenesis,thereby promoted tumor growth and transferring.However,the modified stem cells caused the opposite effects.On the other hand,the stem cells were considered as a substance vector to localize tumor focus,in particular,it will be potential for looking for subclinical focus and distal focus.
ABSTRACT
Objective To observe the effects of arsenic trioxide (As2O3) on T24 bladder cancer cells growth, Caspase-3 gene expression, as well as the weight and volume changes of transplantation tumor of hairless mouse. Methods The cell proliferation inhibition was measured by MTT colorimetric assay. Morphological changes in the cell nucleus was measured by fluorescein stain. Morphologic cell apoptosis was analyzed by FCM. The espression of Caspase-3 was observed by immunocytochemistry staining. The weight and volume changes of transplantation tumor of hairless mouse were measured before and after As2 O3 was used. Results As2 O3 could significantly inhibit the growth of T24 cells, which depended on the reaction time and concentration of drug. With the extension of the time, the increase in the rate of apoptosis and expression of casepase-3 increased. The tumor size and tumor analysis of As2O3 group decreased than those of the control group(P＜0. 05). Conclusion As2O3 could significantly inhibit the growth of bladder cancer cells and its anti-cancer mechanisms may be related to increased Caspase-3 protein expression.